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Related Experiment Video

Updated: Jul 2, 2026

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter
08:29

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

Published on: April 22, 2014

Improved continuous-flow print head for micro-array deposition.

Mark A Eddings1, Adam R Miles, Josh W Eckman

  • 1Department of Bioengineering, University of Utah, Salt Lake City, UT 84132, USA.

Analytical Biochemistry
|August 16, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces a new microfluidic spotter that significantly improves ligand deposition for surface plasmon resonance imaging (SPRi). The enhanced technology requires substantially less sample, expanding SPRi applications in antibody screening and biomarker detection.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Surface Science

Background:

  • Conventional micro-array pin spotting limits surface plasmon resonance imaging (SPRi) applications due to inefficient ligand deposition.
  • There is a need for improved methods to enhance ligand deposition for SPRi technology.

Purpose of the Study:

  • To introduce a modified continuous-flow micro-spotting technology for improved ligand deposition in SPRi.
  • To demonstrate the efficiency and reduced sample requirements of the new microfluidic spotter.

Main Methods:

  • Modification of continuous-flow micro-spotting technology.
  • Utilized Flexchip protein A/G and neutravidin capturing surfaces.
  • Varied deposition flow rate and conducted contact time studies.

Main Results:

  • The new microfluidic spotter requires 1000x less concentrated antibodies and biotinylated ligands compared to pin spotting.
  • Demonstrated efficient sample delivery to the substrate surface via tip overlay flow cell design.
  • Achieved successful capture of antibodies and biotinylated ligands at concentrations as low as 0.1 ug/ml and 100 pM, respectively.

Conclusions:

  • The developed microfluidic spotter significantly enhances ligand deposition efficiency for SPRi.
  • This advancement reduces sample consumption and expands the utility of SPRi systems.
  • Potential applications include antibody screening, carbohydrate arrays, and biomarker detection.