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Related Experiment Video

Updated: Jul 2, 2026

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
12:08

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

Published on: March 10, 2016

Maintaining epitheliopoietic potency when culturing olfactory progenitors.

Woochan Jang1, James Lambropoulos, Jin Kyung Woo

  • 1Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, MA 02111, USA.

Experimental Neurology
|August 16, 2008
PubMed
Summary

A novel air-liquid interface culture system preserves the regenerative potential of olfactory epithelium stem cells. This method enables progenitor cells to form spheres and regenerate diverse cell types in vivo after injury.

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Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • The olfactory epithelium contains multipotent, neurocompetent progenitor and stem cells throughout life.
  • These cells can replace all epithelial cell types after injury, offering therapeutic potential.
  • Developing a culture system to maintain progenitor potency during expansion is crucial for therapeutic applications.

Purpose of the Study:

  • To develop a culture system that maintains the in vivo potency of adult olfactory epithelium progenitor cells.
  • To assess the ability of cultured progenitors to regenerate olfactory epithelium following injury.

Main Methods:

  • Utilized an air-liquid interface culture protocol with a 3T3 feeder cell layer.
  • Seeded FACS-isolated or unsorted progenitor cells from methyl bromide-lesioned adult rodent olfactory epithelium.

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Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
12:17

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells

Published on: January 2, 2016

Related Experiment Videos

Last Updated: Jul 2, 2026

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
12:08

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

Published on: March 10, 2016

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
12:17

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells

Published on: January 2, 2016

  • Organized cells into three-dimensional, epithelium-lined spheres capable of passaging.
  • Transplanted spheres derived from GFP-expressing mice into methyl bromide-lesioned wild-type mice via nasal infusion.

    • Main Results:

    • Epithelial cells formed complex, epithelium-lined spheres containing globose basal cells, horizontal basal cells, sustentacular cells, and neurons.
    • Transplanted spheres successfully regenerated various cell types in vivo, forming functional clones.
    • Two-dimensional submerged cultures resulted in fibroblastic transition and failed integration after transplantation.

    Conclusions:

    • The described air-liquid interface culture system effectively maintains the potency of olfactory epithelium progenitor cells.
    • This system supports in vivo epitheliopoiesis, demonstrating the potential for regenerative therapies.
    • Preserving progenitor cell potency in culture is key for successful olfactory epithelium regeneration.