Related Experiment Video
Updated: Jul 2, 2026

11:34
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
Published on: August 9, 2019
Identification and characterization of PNRC splicing variants
Yuanzhong Wang1, Yuping Li, Bin Chen
1Department of Biochemistry and Molecular Biology, Third Military Medical University, Chonqing, 400038, PR China.
Gene
|August 16, 2008
Summary
Proline-rich nuclear receptor coregulatory protein (PNRC) has distinct isoforms generated by alternative splicing. These PNRC variants modulate nuclear receptor transcription, revealing a novel regulatory mechanism.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Nuclear receptors (NRs) regulate gene transcription via coregulators.
- Proline-rich nuclear receptor coregulatory protein (PNRC) potentiates NR transcriptional activity.
Purpose of the Study:
- Identify and characterize human PNRC splicing variants.
- Investigate the functional differences of PNRC isoforms in NR transcription.
Main Methods:
- Splicing variant identification.
- Protein truncation analysis.
- Nuclear receptor binding assays.
- Transcriptional activity assays.
Main Results:
- Three human PNRC splicing variants (PNRC1c, PNRC1d, PNRC1f) were identified.
- Isoforms exhibit differential NR binding and transcriptional potentiation.
- PNRC1d modulates full-length PNRC activity in estrogen receptor (ER) transcription.
Conclusions:
- PNRC exists as functionally distinct isoforms.
- Alternative splicing regulates PNRC coactivator activity.
- PNRC isoforms play diverse roles in nuclear receptor-mediated gene regulation.
Related Concept Videos
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Comparing Copy Number Variations and SNPs
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
