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Updated: Jul 2, 2026

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
Published on: February 7, 2022
Low-dose aberration corrected cryo-electron microscopy of organic specimens
James E Evans1, Crispin Hetherington, Angus Kirkland
1Molecular and Cellular Biology, University of California, Davis, CA 95616, USA. JEEvans@ucdavis.edu
Abstract:
Spherical aberration (C(s)) correction in the transmission electron microscope has enabled sub-angstrom resolution imaging of inorganic materials. To achieve similar resolution for radiation-sensitive organic materials requires the microscope to be operated under hybrid conditions: low electron dose illumination of the specimen at liquid nitrogen temperature and low defocus values. Initial images from standard inorganic and organic test specimens have indicated that under these conditions C(s)-correction can provide a significant improvement in resolution (to less than 0.16nm) for direct imaging of organic samples.
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