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Updated: Jul 2, 2026

Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass
Published on: April 18, 2020
Value addition to corncob: production and characterization of xylooligosaccharides from alkali pretreated
Ayyappan Appukuttan Aachary1, Siddalingaiya Gurudutt Prapulla
1Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore 570 020, Karnataka, India.
Abstract:
Comparison of various pretreatments such as mild alkali/acid treatments and pressure cooking of corncob to expose its lignin-saccharide complex has been carried out to enhance enzymatic hydrolysis of xylan to xylooligosaccharides (XOS). Scanning electron micrographs of lignin-saccharide complex of native and pretreated corncob powder showed that the complex was greatly altered during alkali pretreatment. Hydrolysis of alkali pretreated corncob powder using a commercial endoxylanase produced 81+/-1.5% of XOS in the hydrolyzate equivalent to 5.8+/-0.14 mg ml(-1) of XOS. Reaction parameters for the production of XOS from corncob using endoxylanase from Aspergillus oryzae MTCC 5154 were optimized and an XOS yield of 10.2+/-0.14 mg ml(-1) corresponding to 81+/-3.9% with 73.5% xylobiose was obtained. HPLC/RID and ESI/MS analysis of XOS mixture and purified fractions showed that XOS was a mixture of neutral oligosaccharides of DP, 2-7.
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