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Related Experiment Video

Updated: Jul 2, 2026

ES Cell-derived Neuroepithelial Cell Cultures
07:45

ES Cell-derived Neuroepithelial Cell Cultures

Published on: November 30, 2006

ES cell-derived neuroepithelial cell cultures.

Shreeya Karki1, Jan Pruszak, Ole Isacson

  • 1McLean Hospital, Harvard Medical School, USA. skarki@mclean.harvard.edu

Journal of Visualized Experiments : Jove
|August 16, 2008
PubMed
Summary

Embryonic stem cells (ES cells) can generate neural precursors for cell therapies. This study details generating neural rosettes using stromal cell-derived inducing activity (SDIA) for efficient neural cell development.

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Area of Science:

  • Stem cell biology
  • Developmental neuroscience
  • Regenerative medicine

Background:

  • Embryonic stem cells (ES cells) offer therapeutic potential due to their pluripotency.
  • ES cell differentiation involves generating progenitor cells that mature into specific cell types.
  • Neurogenesis from ES cells requires deriving and expanding neural precursors before terminal differentiation.

Purpose of the Study:

  • To describe a method for generating and isolating neural progenitor rosettes from ES cells.
  • To investigate the efficiency of stromal cell-derived inducing activity (SDIA) in neural induction.
  • To provide a foundation for developing ES cell-based neural therapies.

Main Methods:

  • Co-culturing ES cells with the MS5 stromal cell line to induce neuroepithelial development.

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Last Updated: Jul 2, 2026

ES Cell-derived Neuroepithelial Cell Cultures
07:45

ES Cell-derived Neuroepithelial Cell Cultures

Published on: November 30, 2006

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery
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An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

Published on: August 23, 2016

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08:55

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay

Published on: February 14, 2025

  • Utilizing stromal cell-derived inducing activity (SDIA) for efficient neural progenitor generation.
  • Formation and isolation of rosette-like structures resembling neural tube progenitors.

    • Main Results:

    • SDIA effectively induces the formation of neuroepithelial precursors from ES cells.
    • Rosette structures, indicative of neural progenitors, were successfully generated and isolated.
    • The described method facilitates the expansion and subsequent differentiation of neural precursors.

    Conclusions:

    • Co-culture with MS5 cells provides an efficient route to generate neural progenitors via SDIA.
    • Isolated neural rosettes serve as a valuable source for deriving specific neuronal and glial subtypes.
    • This approach advances the development of cell-based therapies for neurological disorders.