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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

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Related Experiment Video

Updated: Jul 2, 2026

Freezing Human ES Cells
08:00

Freezing Human ES Cells

Published on: October 12, 2006

Freezing human ES cells.

Erin Trish1, John Dimos, Kevin Eggan

  • 1Dept of Molecular and Cellular Biology, Harvard University, USA. etrish@mcb.harvard.edu

Journal of Visualized Experiments : Jove
|August 16, 2008
PubMed
Summary
This summary is machine-generated.

This study details a reliable method for cryopreserving HuES human embryonic stem cells (hESCs). The protocol ensures successful long-term storage and viability of these valuable stem cell lines.

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Last Updated: Jul 2, 2026

Freezing Human ES Cells
08:00

Freezing Human ES Cells

Published on: October 12, 2006

Freezing and Thawing Human Embryonic Stem Cells
08:49

Freezing and Thawing Human Embryonic Stem Cells

Published on: December 24, 2009

Human ES cells: Starting Culture from Frozen Cells
02:47

Human ES cells: Starting Culture from Frozen Cells

Published on: November 9, 2006

Area of Science:

  • Cell Biology
  • Stem Cell Research
  • Cryobiology

Background:

  • Human embryonic stem cells (hESCs) are crucial for regenerative medicine and disease modeling.
  • Maintaining viable hESC cultures requires robust preservation methods.
  • Established protocols for hESC cryopreservation are essential for research continuity.

Purpose of the Study:

  • To present a standardized laboratory protocol for the cryopreservation of HuES human embryonic stem cell lines.
  • To ensure the long-term viability and genetic integrity of hESCs post-thawing.
  • To provide a reproducible method for researchers working with hESCs.

Main Methods:

  • Washing exponentially expanding hESC cultures with PBS.
  • Detaching cells using 0.05% Trypsin-EDTA at room temperature.
  • Resuspending cells in growth media followed by addition of freezing media.
  • Controlled slow freezing to -80°C over 24 hours.
  • Long-term storage in liquid nitrogen vapor phase or at -80°C.

Main Results:

  • Demonstration of a step-by-step cryopreservation technique for HuES hESCs.
  • Achieved cell viability suitable for resurrecting a full culture from a single aliquot.
  • Established a method for storage up to six months at -80°C or indefinitely in liquid nitrogen.

Conclusions:

  • The described method provides a reliable approach for cryopreserving human embryonic stem cells.
  • This protocol facilitates the long-term storage and accessibility of valuable hESC lines for future research.
  • Successful cryopreservation is key to advancing stem cell research and therapeutic applications.