Capillary electrophoresis-mass spectrometry in urinary proteome analysis: current applications and future...

Area of Science:

• Proteomics
• Biomarker Discovery
• Clinical Medicine

Background:

• Noninvasive disease diagnosis and prognosis remain challenging in clinical medicine.
• Advancements in protein separation and detection enable proteome screening of biological fluids.
• Clinical studies are increasingly focused on identifying sensitive and selective disease biomarkers.

Purpose of the Study:

• To review state-of-the-art proteomic platforms utilizing urine for biomarker discovery.
• To provide an overview of clinical studies employing capillary electrophoresis-mass spectrometry (CE-MS) in urinary proteomics/peptidomics.

Main Methods:

• Focus on proteomic platforms adapted for urine sample analysis.
• Review of clinical studies using CE-MS for urinary proteomics and peptidomics.
• CE-MS enables high-throughput, one-step screening of the urinary small molecular weight proteome.

Main Results:

• CE-MS offers fast, accurate resolution of the urinary proteome.
• The technique is well-suited for large-scale proteomic studies.
• Urinary proteomics can assess systemic diseases beyond renal and urological disorders.

Conclusions:

• Urine is a valuable source for biomarker discovery, diagnosis, staging, and monitoring disease progression.
• CE-MS is a powerful tool for high-throughput urinary proteomic studies.
• Urinary proteomics holds significant potential for assessing a wide range of diseases.