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Updated: Jul 2, 2026
Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery
Published on: August 16, 2019
[Changes of ErbB receptors mRNA expression in left ventricle of myocardial infarction]
Chun Gui1, Jian-an Wang, Na Li
1Department of Cardiology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Objective:
To investigate the changes of ErbB receptors mRNA expression in left ventricle of myocardial infarction and its mechanism.
Methods:
Myocardial infarction was induced by ligation of the left anterior descending coronary artery in male Sprague Dawley(SD) rats. After 1 month, the rats were sacrificed and mRNA was extracted from the left ventricle. mRNA was also extracted from ventricular myocytes of neonatal SD rats which were cultured under serum deprivation and hypoxia for 5 d. Expression of ErbB receptors mRNA were detected by real-time quantitative PCR.
Result:
The mRNA expression of ErbB2 and ErbB4 receptors was significantly down-regulated in the left ventricle of myocardial infarction compared with the normal left ventricle; in contrast, there were no changes in mRNA expression of ErbB3. Serum deprivation and hypoxia in vitro caused significant down-regulation of the mRNA expression of ErbB2 and ErbB4 receptors, but the mRNA expression of ErbB3 was not changed.
Conclusion:
Expression of ErbB2 and ErbB4 receptors mRNA is down-regulated in myocardial infarction, which may results from the hypoxia, deprivation of nutrients and cytokines.
Insights
Myocardial infarction down-regulates ErbB2 and ErbB4 receptor mRNA in the heart. This reduction in ErbB receptor expression may be caused by hypoxia and nutrient deprivation following heart attack.
Area of Science:
- Cardiovascular Biology
- Molecular Cardiology
- ErbB Receptor Signaling
Background:
- Myocardial infarction (MI) significantly impacts cardiac function.
- ErbB receptors play crucial roles in cardiac cell survival and function.
- Understanding changes in ErbB receptor expression post-MI is vital for therapeutic development.
Purpose of the Study:
- To investigate alterations in ErbB receptors mRNA expression in the left ventricle following myocardial infarction.
- To explore the underlying mechanisms contributing to these expression changes.
Main Methods:
- Myocardial infarction induced in Sprague Dawley rats via coronary artery ligation.
- mRNA extracted from left ventricles of MI rats and neonatal rat ventricular myocytes.
- Real-time quantitative PCR used to detect ErbB receptors mRNA expression.
- In vitro model of serum deprivation and hypoxia used.
Main Results:
- Significant down-regulation of ErbB2 and ErbB4 receptor mRNA observed in the left ventricle post-MI.
- No significant change in ErbB3 receptor mRNA expression in MI hearts.
- In vitro, serum deprivation and hypoxia mirrored the down-regulation of ErbB2 and ErbB4 mRNA.
Conclusions:
- ErbB2 and ErbB4 receptor mRNA expression is reduced in myocardial infarction.
- Hypoxia, nutrient deprivation, and cytokines are potential contributors to this down-regulation.
- Findings suggest a role for ErbB signaling in the cardiac response to ischemic injury.
