[Effect of salidroside on salivary adenoid cystic carcinoma cells in vitro]
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Published on: June 9, 2023
Mei-Hua Li1, Gui-Zhen Zhang, Yi-Shu Wang
1Department of Stomatology, The Third Hospital of Jilin University, Changchun 130031, China.
Objective:
To study the effect and mechanism of salidroside on salivary adenoid cystic carcinoma (SACC-2) cells in vitro.
Methods:
To detect the effect of salidroside on SACC-2 cells growth with CCK-8 kit, the growth curve of cells were drawn. To detect the expression of cysteine proteinase (Caspase 3 and Caspase 8) and proliferating cell nuclear antigen (PCNA) in SACC-2 cells, immunohistochemistry staining was used.
Results:
According the results of CCK-8 kit detected, salidroside could inhibit the proliferation of SACC-2 cells, IC50 value was (4.99+/-0.23) microg/mL. Growing curve showed that SACC-2 cells of salidroside groups decreased with extending cell culture time. Immunohistochemistry staining showed that Caspase 3 and Caspase 8 were both strong positive expression in SACC-2 cells of salidroside groups, and poor positive expression in SACC-2 cells of control group, the difference was significant (P<0.01). The expression of PCNA was reverse (P<0.01).
Conclusion:
Salidroside could inhibit the proliferation of SACC-2 cells and induce SACC-2 cell apoptosis in vitro, which could be a kind of antitumor medicine in the future.