A modified CZ-1 preserving solution for organ transplantation: comparative study with UW preserving solution

Area of Science:

• Transplantation Science
• Organ Preservation
• Biomedical Engineering

Background:

• The University of Wisconsin (UW) solution is the gold standard for multiorgan transplantation preservation.
• Limited availability of organ preservation solutions has previously hindered advancements in China's organ transplantation programs.
• This study introduces and validates the Changzheng Organ Preserving Solution (CZ-1) as a potential alternative.

Purpose of the Study:

• To validate the efficacy of the Changzheng Organ Preserving Solution (CZ-1).
• To compare the performance of CZ-1 solution against the established UW solution for organ preservation.
• To assess the feasibility of CZ-1 solution for extended cold storage of various organs.

Main Methods:

• Preservation studies were conducted on kidneys, livers, hearts, lungs, and pancreases from New Zealand rabbits and Sprague-Dawley (SD) rats.
• Organ morphology was evaluated using light and electron microscopy.
• Biochemical functions, physiological viability, and survival rates under prolonged cold storage were assessed.

Main Results:

• CZ-1 solution demonstrated comparable efficacy to UW solution across multiple organs, including rabbit kidneys, livers, hearts, lungs, and rat kidneys, livers, intestinal mucosa, and pancreases.
• CZ-1 exhibited superior preservation in specific cases, such as rabbit hearts and rat intestinal mucosa and pancreases.
• Extended preservation times were achieved with CZ-1: 72 hours for rat kidneys, 24 hours for livers, 18 hours for hearts, and 8 hours for lungs.
• Successful transplantation of 12 kidneys preserved in CZ-1 for 22–31 hours resulted in a mean renal function recovery time of 3.83 days.

Conclusions:

• CZ-1 solution is a viable and effective alternative to UW solution for organ preservation.
• The development of CZ-1 solution offers a cost-effective option, potentially improving organ preservation quality.
• CZ-1 is poised to contribute significantly to the future growth of organ transplantation in China.