Related Experiment Video
Updated: Jul 2, 2026

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
Published on: May 26, 2021
Knockout animals and natural mutations as experimental and diagnostic tool for studying tight junction functions in
1Department of Physiology and Cell Biology, Kobe University, Chuo-ku, Kobe 650-0017, Japan. furuse@med.kobe-u.ac.jp
Abstract:
Two sides of functions of tight junctions; the barrier and the channel in the paracellular pathway are believed to be essential for the development and physiological functions of organs. Recent identification of molecular components of tight junctions has enabled us to analyze their functions by generating knockout mice of the corresponding genes. In addition, positional cloning has identified mutations in the genes of several components of tight junctions in hereditary diseases. These studies have highlighted in vivo functions of tight junctions.
Related Concept Videos
In-vitro Mutagenesis
Tight Junctions
