Interactions of a Rhodococcus sp. biosurfactant trehalose lipid with phosphatidylethanolamine membranes

Trehalose lipids are an important group of glycolipid biosurfasctants mainly produced by rhodococci. Beside their known industrial applications, there is an increasing interest in the use of these biosurfactants as therapeutic agents. We have purified a trehalose lipid from Rhodococcus sp. and made a detailed study of the effect of the glycolipid on the thermotropic and structural properties of phosphatidylethanolamine membranes of different chain length and saturation, using differential scanning calorimetry, small and wide angle X-ray diffraction and infrared spectroscopy. It has been found that trehalose lipid affects the gel to liquid crystalline phase transition of phosphatidylethanolamines, broadening and shifting the transition to lower temperatures. Trehalose lipid does not modify the macroscopic bilayer organization of saturated phosphatidylethanolamines and presents good miscibility both in the gel and the liquid crystalline phases. Infrared experiments evidenced an increase of the hydrocarbon chain conformational disorder and an important dehydrating effect of the interfacial region of the saturated phosphatidylethanolamines. Trehalose lipid, when incorporated into dielaidoylphosphatidylethanolamine, greatly promotes the formation of the inverted hexagonal HII phase. These results support the idea that trehalose lipid incorporates into the phosphatidylethanolamine bilayers and produces structural perturbations which might affect the function of the membrane.