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Updated: Jul 2, 2026
Murine Model of Wound Healing
Published on: May 28, 2013
Homogenous amniotic membrane as a biological dressing for oral mucositis in rats: histomorphometric analysis
Maria das Graças Vilela-Goulart1, Raquel Teodoro Santos Teixeira, Daniela Coelho Rangel
1Bioscience Center for Special Health Care Needs (Centro de Biociências Aplicado a Pacientes com Necessidades Especiais/CEBAPE-UNESP), São José dos Campos Dental School, São Paulo State University-UNESP, São Paulo, Brazil.
Objective:
Oral mucositis is a frequent and significant side effect of chemotherapy and/or radiation therapy. The purpose of this study was to evaluate the effects of the homogenous amniotic membrane (HAM) as a biological dressing for oral mucositis induced by 5-fluorouracil (5-FU) in the labial fornix region of inferior incisors in rats.
Methods:
Sixty rats were divided into three groups: control (50% acetic acid), 5-FU (50% acetic acid and 5-fluorouracil-induced oral mucositis) and 5-FU+HAM (50% acetic acid and 5-fluorouracil-induced oral mucositis, and treated with HAM). Histological analyses were done 3, 7, 14 and 21 days after applying the acetic acid.
Results:
HAM showed not only no signs of rejection as well as an excellent tissue adherence to the ulcerated surface. Histomorphometric analysis indicated a lower quantity of inflammatory cells in the 5-FU+HAM group in all periods when compared to the other groups and there was a statistically significant difference in the 3- and 7-day periods (P<0.001).
Conclusions:
The results of this study suggest that HAM was biocompatible with great adhesive power to the ulcerated surface, accelerated the healing process and exhibited anti-inflammatory activity. Further studies should be done to investigate the potential benefits of this material in clinical trials. HAM could lead to improved results as a biological dressing in treatment and management of oral mucositis in patients submitted to chemotherapy and/or radiation therapy.
