Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 2, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

Functional whole-colony screening method to identify antimicrobial peptides.

E Loit1, K Wu, X Cheng

  • 1Centre for Research on Environmental Microbiology, Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, Canada.

Journal of Microbiological Methods
|August 19, 2008
PubMed
Summary

A novel high-throughput screening method identifies antimicrobial peptides (AMPs) using a vital dye assay. This technique rapidly detects peptides that compromise bacterial viability, accelerating the discovery of new antimicrobial agents.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Research progress on clinical phenotyping and related mechanism between SCN10A/Nav1.8 and cardiovascular disease].

Zhonghua xin xue guan bing za zhi·2021
Same author

Morroniside suppresses hydrogen peroxide-stimulated autophagy and apoptosis in rat ovarian granulosa cells through the PI3K/AKT/mTOR pathway.

Human & experimental toxicology·2020
Same author

[Clinical profile of pheochromocytoma and paraganglioma with normal plasma free metanephrines].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2020
Same author

Tailoring phase transformation strengthening and plasticity of nanostructured high entropy alloys.

Nanoscale·2020
Same author

Gene polymorphisms of pro-inflammatory cytokines may affect the risk of Graves' disease: a meta-analysis.

Journal of endocrinological investigation·2020
Same author

[Comparison of short-term and long-term efficacy between robot-assisted and thoracoscopy-laparoscopy-assisted radical esophageal cancer surgery].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2020

Area of Science:

  • Biotechnology
  • Microbiology
  • Drug Discovery

Background:

  • Antimicrobial peptides (AMPs) are crucial in innate immunity.
  • Discovering novel AMPs is essential to combat rising antibiotic resistance.
  • Existing screening methods can be time-consuming and low-throughput.

Purpose of the Study:

  • To develop and validate a high-throughput screening method for identifying antimicrobial peptides (AMPs).
  • To assess the efficacy of a novel dye inclusion assay for detecting bacterial viability antagonism.
  • To screen a synthetic oligonucleotide library for novel AMPs.

Main Methods:

  • A high-throughput method using a vital dye inclusion assay was developed.
  • Colonies were grown on a membrane and transferred to an inductive medium with vital dye.

More Related Videos

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
08:54

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds

Published on: February 14, 2018

Related Experiment Videos

Last Updated: Jul 2, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
08:54

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds

Published on: February 14, 2018

  • Compromised cell membranes due to peptide expression allowed dye infusion for visual identification.

    • Main Results:

    • The method successfully identified three antimicrobial peptides (L1, L3, L8) from a library of 100,000 potential peptides.
    • Peptide L1 exhibited concentration-dependent bactericidal effects against Gram-negative bacteria and biostatic activity against Gram-positive bacteria.
    • Peptide L8 showed bacteriostatic and potentially bactericidal effects against multiple Gram-negative bacteria.

    Conclusions:

    • The developed high-throughput assay based on filter-bound colony arrays and vital dye inclusion is effective for AMP identification.
    • This method accelerates the discovery of novel antimicrobial peptides.
    • The identified peptides L1 and L8 demonstrate significant antimicrobial potential.