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Selenium concentration in ocular tissues and fluids.

M C McGahan1, A M Grimes

  • 1Department of Anatomy, College of Veterinary Medicine, North Carolina State University, Raleigh.

Ophthalmic Research
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

This study presents a precise method for analyzing selenium (Se) in eye tissues and fluids. Selenium levels in ocular tissues were higher than in aqueous humor, suggesting protein binding influences its distribution.

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Area of Science:

  • Ophthalmology
  • Analytical Chemistry
  • Toxicology

Background:

  • Selenium (Se) is an essential trace element with vital roles in ocular health.
  • Understanding Se distribution in ocular tissues and fluids is crucial for diagnosing and treating related conditions.
  • Previous methods for Se analysis in biological samples were complex or lacked precision.

Purpose of the Study:

  • To develop and validate a simple, precise method for quantifying selenium in ocular fluids and tissues.
  • To determine the concentration of Se in various ocular compartments (cornea, iris, lens, retina, aqueous humor).
  • To investigate the relationship between Se levels, protein concentration, and its distribution between plasma and aqueous humor.

Main Methods:

  • Electrothermal atomic absorption spectroscopy (ETAAS) was employed for Se analysis.
  • Ocular tissues (cornea, iris, lens, retina) and aqueous humor were collected from subjects.
  • Se concentrations were measured and compared with plasma Se levels and protein concentrations.

Main Results:

  • Se concentrations in ocular tissues ranged from 0.23 to 0.41 microgram/g wet weight.
  • Aqueous humor exhibited significantly lower Se concentrations (0.008 microgram/ml) compared to plasma (0.21 microgram/ml).
  • Aqueous humor protein concentration was approximately 1.0% of plasma protein concentration.

Conclusions:

  • The developed ETAAS method provides a precise and simple approach for ocular Se analysis.
  • Selenium distribution in the eye is influenced by protein binding, as evidenced by lower Se levels in protein-poor aqueous humor compared to plasma.
  • Further research into Se's role in ocular health and disease is warranted based on these findings.