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Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging.

Tom Robinson1, Prashant Valluri, Hugh B Manning

  • 1Chemical Biology Centre, Department of Chemical Engineering, Imperial College London, London, UK.

Optics Letters
|August 19, 2008
PubMed
Summary

Fluorescence lifetime imaging (FLIM) quantitatively mapped small molecule concentration in a microfluidic device. Optimized mixing achieved times as fast as 1.3 ms, validated by computational fluid-dynamics (CFD) simulations.

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Area of Science:

  • Microfluidics
  • Analytical Chemistry
  • Biophysics

Background:

  • Quantitative analysis of small molecule concentration in microfluidic devices is crucial for process optimization.
  • Fluorescence lifetime imaging (FLIM) offers a non-invasive method for molecular mapping.
  • Computational fluid-dynamics (CFD) simulations are valuable tools for predicting fluid behavior in microchannels.

Purpose of the Study:

  • To quantitatively map the 3D concentration of a small molecule within a microfluidic mixing device.
  • To compare experimental FLIM data with CFD simulations for validation and optimization.
  • To achieve optimized, rapid mixing in a single-layer microfluidic device.

Main Methods:

  • Utilized a line-scanning semiconfocal FLIM microscope for high-resolution imaging.
  • Performed 3D concentration mapping of a small molecule.
  • Employed CFD simulations to model and optimize fluid dynamics.
  • Integrated experimental FLIM data with CFD results.

Main Results:

  • Achieved submicrometer resolution imaging of the full mixing profile in a single scan.
  • Experimental data were in good agreement with CFD simulations.
  • Optimized microfluidic device design led to mixing times as low as 1.3 ± 0.4 ms.

Conclusions:

  • FLIM is an effective technique for quantitative 3D molecular mapping in microfluidic systems.
  • The combination of FLIM and CFD enables efficient optimization of microfluidic mixing.
  • Rapid mixing times were successfully demonstrated in a simplified microfluidic device architecture.