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Related Experiment Video

Updated: Jul 2, 2026

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

[A new method for SNP typing based on allele specific PCR].

Rui-Heng Wang¹, Li-Min Liu, Jin-Ling Zhao

¹School of Forensic Medicine, China Medical University, Shenyang 110001, China. dalianwrh@sina.com

Fa Yi Xue Za Zhi

|August 20, 2008

Summary

This study introduces a new SNP typing method using allele-specific PCR and capillary electrophoresis. The fragment length discrepant allele specific fluorescence labeled multi-PCR (FLDASFLM-PCR) method efficiently genotypes multiple SNPs simultaneously.

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Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Area of Science:

Molecular Biology
Genetics
Biotechnology

Context:

Single Nucleotide Polymorphisms (SNPs) are crucial genetic markers.
Accurate and efficient SNP typing is essential for genetic research and diagnostics.
Existing methods may have limitations in throughput or cost.

Purpose:

To develop a novel, efficient method for SNP typing.
To leverage allele-specific PCR and capillary electrophoresis for simultaneous multi-SNP analysis.
To establish a robust assay for genotyping multiple diallelic SNP loci.

Summary:

A new method, FLDASFLM-PCR, was developed using allele-specific primers and capillary electrophoresis.
Two forward primers of different lengths, with an artificial mismatch, were designed for each SNP.
Simultaneous amplification and analysis of 11 SNP loci were achieved, with results validated by direct sequencing.

Impact:

FLDASFLM-PCR offers a simple, rapid, and efficient approach for SNP typing.
This method facilitates high-throughput genotyping for genetic studies.
The technique provides accurate genotype determination for multiple SNPs in a single reaction.