Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Actin Polymerization and Cell Motility

Actin Polymerization and Cell Motility

Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.

Cytoskeletal Coordination in Cell Migration

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...

Chemotaxis and Direction of Cell Migration

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon towards...

Detailed Structure and Function of Lymph Nodes

Detailed Structure and Function of Lymph Nodes

Lymph nodes are bean-shaped structures that cluster along the lymphatic vessels in the inguinal, axillary, and cervical regions. Each node is divided into compartments by a capsule that extends trabeculae inward.
From a histological perspective, lymph nodes can be split into two main areas: the superficial cortex and the deep medulla. The outer cortex is populated by dendritic cells, macrophages, and B lymphocytes, which are densely packed into follicles. When these B-lymphocytes are presented...

Cell Migration

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.

Cell Migration

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CXCR3 is associated with T-cell-induced heart damage in acute rheumatic fever.

Nature communications·2026

Same author

Enhanced HDR-mediated correction of heterozygous <i>COL7A1</i> mutations for recessive dystrophic epidermolysis bullosa.

Molecular therapy. Nucleic acids·2025

Same author

A novel approach to digital characterisation of Tertiary Lymphoid Structures in colorectal cancer.

Frontiers in immunology·2025

Same author

Efficient Dual Cas9 Nickase Correction of a Prevalent Pathogenic <i>LAMB</i> <i>3</i> Variant for Junctional Epidermolysis Bullosa.

JID innovations : skin science from molecules to population health·2025

Same author

Human keratinocytes grown at a gas-permeable interface in vitro stratify correctly to generate engineered human epidermis.

Cytotherapy·2025

Same author

Proliferating macrophages in human tumours show characteristics of monocytes responding to myelopoietic growth factors.

Frontiers in immunology·2024

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes

Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes

Published on: July 15, 2011

Simulating T-cell motility in the lymph node paracortex with a packed lattice geometry.

Gib Bogle¹, P Rod Dunbar

¹Maurice Wilkins Centre, University of Auckland, Auckland, New Zealand. g.bogle@auckland.ac.nz

Immunology and Cell Biology

|August 20, 2008

Summary

A new computational model simulates T-cell movement in lymph nodes. This lattice-based model accurately reproduces T-cell random walks and is efficient for large-scale simulations.

More Related Videos

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Published on: June 1, 2010

Real-Time In Vitro Migration Assay for Primary Murine CD8+ T Cells

Real-Time In Vitro Migration Assay for Primary Murine CD8+ T Cells

Published on: May 24, 2024

Related Experiment Videos

Last Updated: Jul 2, 2026

Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes

Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes

Published on: July 15, 2011

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Published on: June 1, 2010

Real-Time In Vitro Migration Assay for Primary Murine CD8+ T Cells

Real-Time In Vitro Migration Assay for Primary Murine CD8+ T Cells

Published on: May 24, 2024

Area of Science:

Immunology
Computational Biology
Biophysics

Background:

Simulating T-cell dynamics in lymph nodes requires accurate cell motility models.
Existing models struggle with T-cell random-walk behavior, cell packing, and computational efficiency.

Purpose of the Study:

To develop a computationally efficient motility model for T-cells within the lymph node paracortex.
To accurately replicate the in vivo random-walk behavior of T-cells.

Main Methods:

Developed a 3D lattice-based geometry for agent-based simulation.
Implemented discrete cell jumps with probabilistic direction determination.
Defined model parameters to capture T-cell movement characteristics.

Main Results:

The model successfully reproduces the characteristic random-walk motion of T-cells.
The lattice-based approach accommodates densely packed cells.
The model demonstrates high computational efficiency.

Conclusions:

The developed motility model is suitable for simulating T-cell populations in the lymph node paracortex.
This model provides an efficient and accurate tool for immunological simulations.
It advances agent-based modeling for studying T-cell trafficking and activation.