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Stimulation of leukocyte lysophospholipase activity by noninfectious agents
1Department of Microbiology, Southeastern University of the Health Sciences, North Miami Beach, Florida 33162.
Abstract:
Mouse peritoneal leukocyte lysophospholipase (LPL) activity was studied to determine whether or not noninfectious agents cause increased enzyme activity and whether neutrophils have LPL activity. In the first study, mice infected with Ascaris suum, a known inducer of LPL activity, were given intraperitoneal injections of proteose peptone, thioglycolate, bovine albumin, paraffin, glycogen, or A. suum whole worm extract (WWE). Cell populations collected from mice injected with A. suum WWE, proteose peptone, thioglycolate, or bovine albumin contained increased numbers of neutrophils and eosinophils. These cell populations had increased LPL activity when treated, in vitro, with either A. suum WWE, zymosan-activated complement, or with the agent they were induced with. However, the LPL activity of the different cell populations did not respond to all treatments in the same way. In a second study, A. suum-infected or noninfected mice were given intraperitoneal injections of paraffin, thioglycolate, glycogen, or A. suum WWE. Enriched cell populations containing either lymphocytes or macrophages, from infected or noninfected mice, did not have increased LPL activity following in vitro stimulation with A. suum WWE, zymosan-activated complement, or with the agent they were induced with. Enriched neutrophil populations from infected or noninfected mice had increased LPL activity following in vitro treatment with A. suum WWE or zymosan-activated complement. Results demonstrate that the LPL activity of peritoneal leukocytes can be induced by noninfectious agents and that neutrophils have increased LPL activity following in vitro stimulation.
Insights
Noninfectious agents can increase mouse leukocyte lysophospholipase (LPL) activity. Neutrophils, but not lymphocytes or macrophages, show enhanced LPL activity after stimulation with specific agents.
Area of Science:
- Immunology
- Biochemistry
Background:
- Lysophospholipase (LPL) activity in mouse peritoneal leukocytes is not fully understood.
- Investigating the role of noninfectious agents in modulating LPL activity is crucial for understanding immune responses.
Purpose of the Study:
- To determine if noninfectious agents can induce increased lysophospholipase (LPL) activity in mouse peritoneal leukocytes.
- To ascertain whether neutrophils possess LPL activity and if it can be modulated.
Main Methods:
- Mice were injected with various noninfectious agents (proteose peptone, thioglycolate, etc.) or Ascaris suum whole worm extract (WWE).
- Peritoneal leukocytes were isolated, and cell populations (neutrophils, eosinophils, lymphocytes, macrophages) were analyzed for LPL activity after in vitro stimulation.
- Specific agents used for induction included A. suum WWE, zymosan-activated complement, and the inducing agent itself.
Main Results:
- Noninfectious agents like proteose peptone, thioglycolate, and bovine albumin increased neutrophil and eosinophil counts and LPL activity.
- Enriched neutrophil populations from both infected and noninfected mice showed increased LPL activity upon in vitro stimulation with A. suum WWE or zymosan-activated complement.
- Lymphocyte and macrophage populations did not exhibit increased LPL activity under similar stimulation conditions.
Conclusions:
- Peritoneal leukocyte lysophospholipase (LPL) activity can be induced by noninfectious agents.
- Neutrophils are a key cell type exhibiting increased LPL activity following in vitro stimulation, suggesting their involvement in inflammatory processes.