APE1 and XRCC3 polymorphisms and myocardial infarction
Atike Tekeli1, Selim Isbir, Arzu Ergen
1Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Capa-Istanbul, Turkey.
Background:
In most cells, DNA is regularly damaged by mutagens. Different DNA repair mechanisms operate on specific types of damaged DNA. When DNA damage resulting from free radicals is not repaired, it might lead to deteriorated gene expression, the development of a number of diseases such as cancer, diabetes, vascular diseases, and aging. In the present study, APE1 and XRCC3 gene polymorphisms were investigated in patients with myocardial infarction.
Materials And Methods:
Forty-five first time elective coronary artery bypass grafting (CABG) patients with cardiopulmonary bypass (CPB) and 40 healthy individuals were studied. Gene polymorphisms were determined by a polymerase chain reaction-restriction fragment length polymorphism method.
Results:
For the APE1 gene, the AG genotype was significantly higher in the patient group than in the control group. The patient group had significantly more G carriers but there was no statistically significant difference between patient and control groups the A allele. The XRCC3 TT genotype was found to be significantly more frequent in the patient group than it was in the control group.
Conclusion:
The results of our study suggested that the XRCC3 gene TT genotype and the APE1 gene AG genotype might increase the risk of myocardial infarcts.
Insights
Genetic variations in the AP endonuclease 1 (APE1) and X-ray repair cross-complementing protein 3 (XRCC3) genes may increase myocardial infarction risk. Specifically, the APE1 AG genotype and XRCC3 TT genotype were more prevalent in patients with heart attacks.
Area of Science:
- Genetics
- Molecular Biology
- Cardiovascular Disease
Background:
- DNA damage from mutagens is common and can lead to diseases like cancer and aging if unrepaired.
- Specific DNA repair mechanisms target distinct types of DNA damage.
- Unrepaired free radical DNA damage is linked to gene expression decline and various pathologies.
Purpose of the Study:
- To investigate the association between APE1 and XRCC3 gene polymorphisms and myocardial infarction (MI).
Main Methods:
- Study included 45 patients undergoing coronary artery bypass grafting (CABG) and 40 healthy controls.
- Gene polymorphisms were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.
Main Results:
- The APE1 gene AG genotype was significantly more frequent in MI patients compared to controls.
- A higher prevalence of G carriers for the APE1 gene was observed in the patient group.
- The XRCC3 gene TT genotype was significantly more frequent in the MI patient group.
Conclusions:
- The XRCC3 TT genotype and APE1 AG genotype may be associated with an increased risk of myocardial infarction.
- These genetic polymorphisms could serve as potential biomarkers for cardiovascular disease risk assessment.
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