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Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

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Variable volume fed-batch fermentation for nisin production by Lactococcus lactis subsp. lactis W28.

Zhaoliang Wu¹, Lin Wang, Yingjun Jing

¹Department of Bioengineering, Hebei University of Technology, Tianjin 300132, China. zhaoliangwu@163.com

Applied Biochemistry and Biotechnology

|August 21, 2008

Summary

Optimized fed-batch fermentation significantly boosts nisin production by Lactococcus lactis. Variable volume intermittent fed-batch fermentation (VVIF) enhances biomass and nisin yield by over 60% compared to traditional batch methods.

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Area of Science:

Microbiology
Biotechnology
Fermentation Technology

Background:

Nisin, an antimicrobial peptide, is produced by Lactococcus lactis.
Optimizing fermentation processes is crucial for enhancing nisin yield.
Substrate inhibition can limit microbial growth and product formation.

Purpose of the Study:

To establish an effective feeding technology for improving nisin production by Lactococcus lactis subsp. lactis W28.
To investigate the impact of initial sucrose concentration, feeding strategy, and feeding rate on fermentation performance.
To compare the efficacy of fed-batch and variable volume intermittent fed-batch fermentation (VVIF) against traditional batch fermentation.

Main Methods:

Fed-batch fermentation with controlled sucrose feeding.
Variable Volume Intermittent Fed-Batch Fermentation (VVIF) to maintain optimal sucrose levels.
Comparison of biomass and nisin yield under different fermentation strategies.

Main Results:

Fed-batch culture with specific feeding parameters (ISC=10 g/l, 100 ml sucrose at 190 g/l fed at 9-10 ml/h after 3h) increased biomass by 23% and nisin yield by 51% over batch fermentation.
VVIF, maintaining sucrose at 5-10 g/l with ISC=10 g/l, resulted in a 29% increase in biomass and a 60% increase in nisin yield compared to batch fermentation.
VVIF effectively mitigated substrate inhibition by maintaining optimal sucrose concentrations.

Conclusions:

Fed-batch fermentation, particularly VVIF, offers significant improvements in biomass and nisin production by Lactococcus lactis.
VVIF is a scalable and effective method for overcoming substrate inhibition and maximizing nisin yield.
The established feeding technology holds promise for industrial-scale nisin production.