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Updated: Jul 2, 2026

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
Published on: January 5, 2024
Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages
V Suárez1, M Zago, A Quiberoni
1Instituto de Lactología Industrial (INLAIN), Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, Argentina. vivisuar@fiq.unl.edu.ar
Aims:
Frequency of lysogeny in Lactobacillus delbrueckii strains (from commercial and natural starters) and preliminary characterization of temperate bacteriophages isolated from them.
Methods And Results:
Induction of strains (a total of 16) was made using mitomycin C (MC) (0.5 mug ml(-1)). For 37% of the MC-treated supernatants, it was possible to detect phage particles or presence of killing activity, but only two active bacteriophages were isolated. The two temperate phages isolated were prolate-headed phages which belonged to group c of Lact. delbrueckii bacteriophages classification. Different DNA restriction patterns were obtained for each phage, while the structural protein profiles and packaging sites were identical. Distinctive one-step growth curves were exhibited by each phage. An influence of calcium ions was observed for their lysis in broth but not on the adsorption levels.
Conclusions:
Our study showed that lysogeny is also present in Lact. delbrueckii strains, including commercial strains.
Significance And Impact Of The Study:
Commercial strains could be lysogenic and this fact has a great practical importance since they could contribute to the dissemination of active-phage particles in industrial environments.
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