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Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
Published on: May 27, 2019
Characterization of an extracellular polysaccharide produced by a Pseudomonas strain grown on glycerol
Filomena Freitas1, Vitor D Alves, Joana Pais
1CQFB/REQUIMTE, Chemistry Department, FCT/Universidade Nova de Lisboa, Caparica 2829-516, Portugal.
Abstract:
A new extracellular charged polysaccharide composed mainly by galactose, with lower amounts of mannose, glucose and rhamnose, was produced by the cultivation of Pseudomonas oleovorans NRRL B-14682 using glycerol as the sole carbon source. Thermal and solid-state NMR analysis showed that this polymer is essentially amorphous, with a glass transition temperature of 155.7 degrees C. The exopolysaccharide aqueous solutions have viscoelastic properties similar to that of Guar gum, but with affinity to salts as a result of its polyelectrolyte character. In addition, the exopolysaccharide has demonstrated good flocculating and emulsifying properties and film-forming capacity. These properties make this polymer a good alternative to more expensive natural polysaccharides, such as Guar gum, in several applications in the food, pharmaceutical, cosmetic, textile, paper and petroleum industries.
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