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Updated: Jul 2, 2026
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
Published on: December 2, 2018
Effect of electromagnetic pulse exposure on permeability of blood-testicle barrier in mice
Xiao-Wu Wang1, Gui-Rong Ding, Chang-Hong Shi
1Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, Shaanxi, China.
Objective:
To study the effect of electromagnetic pulse (EMP) exposure on the permeability of blood-testicle barrier (BTB) in mice.
Methods:
Adult male BALB/c mice were exposed to EMP at 200 kV/m for 200 pulses with 2 seconds interval. The mice were injected with 2% Evans Blue solution through caudal vein at different time points after exposure, and the permeability of BTB was monitored using a fluorescence microscope. The testis sample for the transmission electron microscopy was prepared at 2 h after EMP exposure. The permeability of BTB in mice was observed by using Evans Blue tracer and lanthanum nitrate tracer.
Results:
After exposure, cloudy Evans Blue was found in the testicle convoluted seminiferous tubule of mice. Lanthanum nitrate was observed not only between testicle spermatogonia near seminiferous tubule wall and sertoli cells, but also between sertoli cells and primary spermatocyte or secondary spermatocyte. In contrast, lanthanum nitrate in control group was only found in the testicle sertoli cells between seminiferous tubule and near seminiferous tubule wall.
Conclusion:
EMP exposure could increase the permeability of BTB in the mice.
