Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Conductive Fibers of Chitosan/DNA Interfacial Polyelectrolyte Complexation Incorporating Carbon Nanotubes.

ACS applied materials & interfaces·2026

Same author

Nondestructive In-Situ Measurement of Alkaline Phosphatase Activity of Human Intestinal Organoids in Hydrogel Domes Using Scanning Electrochemical Microscopy.

Analytical chemistry·2026

Same author

Photoelectrochemical bioimaging of redox events for 3D cultured cells via hematite-based light-addressable electrodes.

Biosensors & bioelectronics·2026

Same author

Genomic characterization of <i>bla</i> <sub>OXA-48</sub> -carrying IncL plasmids in <i>Enterobacterales</i> in Vietnam.

microPublication biology·2025

Same author

Domain-Specific Simulated Data Enhances Knife-Mark Noise Suppression in Microscopy Images of Materials.

Microscopy (Oxford, England)·2025

Same author

Comparison of microscopic and 3D exoscopic canal wall down tympanomastoidectomy for primary middle ear cholesteatoma.

Acta oto-laryngologica·2025

Same journal

Recent developments of textile-based triboelectric nanogenerators for smart sports applications.

Biosensors & bioelectronics·2026

Same journal

One-Tube RPA-CRISPR-Cas13a assay with rational design for single-molecule detection of waterborne viruses in drinking water treatment.

Biosensors & bioelectronics·2026

Same journal

AI-driven photophysics-aware design of fluorescent probes with applications in α-synuclein biosensing and inhibitor screening.

Biosensors & bioelectronics·2026

Same journal

Three-dimensional helical integration of high-density linear microelectrode arrays and their cross-tissue applications.

Biosensors & bioelectronics·2026

Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026

Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

Negative dielectrophoretic patterning with different cell types.

Masato Suzuki¹, Tomoyuki Yasukawa, Hitoshi Shiku

¹Graduate School of Environmental Studies, Tohoku University 6-6-11-604, Aramaki-Aoba, Aoba, Sendai 980-8579 Japan.

Biosensors & Bioelectronics

|August 22, 2008

Summary

This summary is machine-generated.

This study introduces a new method using negative dielectrophoresis (n-DEP) to pattern multiple cell types on slides without pretreatment. This technique enables rapid, precise cellular micropatterning for cell biology research.

More Related Videos

A Versatile Method of Patterning Proteins and Cells

A Versatile Method of Patterning Proteins and Cells

Published on: February 26, 2017

Cell Patterning Using Magnetic-Archimedes Strategy

Cell Patterning Using Magnetic-Archimedes Strategy

Published on: February 2, 2024

Related Experiment Videos

Last Updated: Jul 2, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

A Versatile Method of Patterning Proteins and Cells

A Versatile Method of Patterning Proteins and Cells

Published on: February 26, 2017

Cell Patterning Using Magnetic-Archimedes Strategy

Cell Patterning Using Magnetic-Archimedes Strategy

Published on: February 2, 2024

Area of Science:

Biotechnology
Cell Biology
Microfluidics

Background:

Cellular patterning is crucial for studying cell-cell interactions.
Existing methods often require slide pretreatment or lack precision.

Purpose of the Study:

To develop a novel, rapid method for patterning multiple cell types using negative dielectrophoresis (n-DEP).
To create cellular micropatterns without special slide pretreatment for fundamental cell biology studies.

Main Methods:

Fabrication of an interdigitated array (IDA) electrode using indium-tin-oxide (ITO).
Application of AC voltage to induce n-DEP, directing cells to low electric field regions.
Sequential patterning of two cell types onto the culture slide.

Main Results:

Successful alignment and adsorption of C2C12 cells within 1-5 minutes using n-DEP.
Creation of periodic and alternate cell lines incorporating two distinct cell types.
Demonstration of a complete two-cell-type patterning process in just 15 minutes.

Conclusions:

The n-DEP method offers a rapid, versatile tool for fabricating complex cellular micropatterns.
This technique facilitates fundamental cell biology research by enabling studies of cell-cell interactions.
The described approach eliminates the need for slide pretreatment, simplifying the patterning process.