Age-related remodeling of glutamic-acid decarboxylase-labeled elements in deafferented piriform cortex of rats

Olfactory bulb (OB) removal has been shown to result in plasticity in the piriform cortex (PC) that is age dependent. We are studying this phenomenon using immunoelectron microscopy of glutamic acid decarboxylase immunoreactivity (GAD, the enzymatic precursor for GABA) at selected postnatal ages and in adults with emphasis on short survival times of 4-7 days after OB ablation. Normally GAD-labeled synaptic terminals form type II symmetric contacts onto unlabeled dendrites and GAD-labeled dendrites receive type I, asymmetric contacts from unlabeled terminals (Westenbroek, et al., 1988a). The OB lesion results in degenerating terminals with type I contacts onto unlabeled and onto GAD-labeled dendrites. Type I postsynaptic sites may be seen partially contacted by or entirely devoid of degenerating terminals and occasionally may be apposed to variable degrees by normal unlabeled or by GAD-positive terminals. Subsequently, some GAD-labeled terminals may form asymmetric type I contacts usually with unlabeled dendrites and rarely with GAD-labeled dendrites. The findings are most common in the youngest subjects and essentially absent in the adult subjects. A sequence of reinnervation of deafferented type I sites by GAD-labeled terminals is suggested for the formation of this "atypical" synapse and the sequelae of this reorganization are discussed.