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Updated: Jul 2, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
Published on: August 22, 2019
Exploring the nature of photo-damage in two-photon excitation by fluorescence intensity modulation
Arijit Kumar De1, Debabrata Goswami
1Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, UP-208016, India.
Abstract:
We investigate the relative photo-damage effects during one- and two-photon excitations and demonstrate that there exist fundamental differences in the damage induced by a high repetition rate laser as compared to that of a CW laser. This difference is evident from the degree of enhanced fluorescence intensity achieved by blanking the excitation with an optical chopper. Such an enhancement in fluorescence intensity provides better signal-to-noise ratio that could have immediate applications in multiphoton imaging of live specimens.
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