Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Diabetic Foot Ulcer

Diabetic Foot Ulcer

Definition A diabetic foot ulcer (DFU) is a chronic, non-healing wound that develops in individuals with diabetes. It typically occurs on pressure-bearing areas such as the heel, metatarsal heads, or hallux, and carries a high risk of infection and amputation.Pathophysiology • The development of DFUs can be explained by four interconnected mechanisms: neuropathy, ischemia, infection, and impaired wound healing. • Neuropathy is the most common factor. Sensory neuropathy reduces pain perception,...

Complications of Diabetes Mellitus

Complications of Diabetes Mellitus

Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia due to insulin deficiency, resistance, or both. Prolonged hyperglycemia disrupts metabolic homeostasis and leads to acute and chronic complications.Acute ComplicationsAcute complications result from sudden metabolic imbalance.Diabetic ketoacidosis (DKA) mainly appears in type 1 diabetes but may also develop in type 2 diabetes, particularly under extreme stress. It arises from severe insulin deficiency,...

Type I Diabetes III: Clinical Manifestations

Type I Diabetes III: Clinical Manifestations

Type 1 diabetes mellitus typically presents with rapid-onset symptoms due to the body’s inability to utilize glucose in the absence of insulin. Since insulin is required for glucose uptake into cells, its deficiency leads to hyperglycemia and cellular energy deprivation, resulting in characteristic clinical features.Polyuria and PolydipsiaOne of the earliest, most prominent symptoms is polyuria (excessive urination). When blood glucose concentrations rise above the renal threshold, the kidneys...

Hyperglycemia

Hyperglycemia

Hyperglycemia is an abnormally high blood glucose level. It is diagnosed by fasting glucose ≥126 mg/dL, 2-hour oral glucose tolerance test (or OGTT) ≥200 mg/dL, random glucose ≥200 mg/dL with symptoms, or HbA1c ≥6.5%. However, HbA1c results may be unreliable in certain conditions, such as anemia or hemoglobinopathies, and the diagnosis should be confirmed unless classic symptoms are present. Postprandial hyperglycemia is typically considered significant when glucose levels exceed 180 mg/dL two...

Diabetic Neuropathy

Diabetic Neuropathy

DefinitionDiabetic neuropathy is nerve damage caused by long-standing diabetes mellitus. It results directly from prolonged high blood sugar levels.PathophysiologyThe pathophysiology of diabetic neuropathy involves both metabolic and vascular disturbances triggered by chronic hyperglycemia.Metabolic injury: Elevated glucose levels activate the polyol pathway within nerve cells, leading to the accumulation of sorbitol and fructose. This increases oxidative stress, disrupts normal nerve...

Hypoglycemia

Hypoglycemia

Hypoglycemia is a blood glucose level below 70 mg/dL. It commonly occurs in individuals using insulin or insulin-secreting drugs, but may also arise in non-diabetic conditions. People with type 1 diabetes are at the highest risk because they depend on exogenous insulin. People with type 2 diabetes are also at risk, especially when treated with insulin or medications such as sulfonylureas, which increase insulin release regardless of blood glucose levels. It develops when insulin levels exceed...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Secondary syphilis resembling lymphoma-like nodules in a 59-year-old woman.

Journal of the European Academy of Dermatology and Venereology : JEADV·2022

Same author

Dupilumab treatment of eczema in a child with STAT3 hyper-immunoglobulin E syndrome.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021

Same author

Association of depression and diabetes complications and mortality: a population-based cohort study.

Epidemiology and psychiatric sciences·2020

Same author

Cutaneous chromoblastomycosis effectively treated with local heat monotherapy.

Clinical and experimental dermatology·2018

Same author

Comparative effect of antipsychotics on risk of self-harm among patients with schizophrenia.

Acta psychiatrica Scandinavica·2018

Same author

Risk profiles of personality traits for suicidality among mood disorder patients and community controls.

Acta psychiatrica Scandinavica·2017

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

Published on: February 17, 2023

Hyperglycaemic conditions decrease cultured keratinocyte mobility: implications for impaired wound healing in

C-C E Lan¹, I-H Liu, A-H Fang

¹Department of Dermatology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

The British Journal of Dermatology

|August 23, 2008

Summary

High blood glucose impairs keratinocyte migration and proliferation, crucial for wound healing in diabetes mellitus (DM). This study explains why diabetic patients experience poor wound healing due to these cellular dysfunctions.

More Related Videos

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

Related Experiment Videos

Last Updated: Jul 2, 2026

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

Published on: February 17, 2023

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

Area of Science:

Cell Biology
Dermatology
Endocrinology

Background:

Diabetes mellitus (DM) is linked to poor wound healing, a complication stemming from elevated blood glucose levels.
Keratinocyte migration and proliferation are essential for proper re-epithelialization during wound repair.
This study investigates the hypothesis that impaired wound healing in DM patients is due to disrupted keratinocyte re-epithelialization.

Purpose of the Study:

To investigate the impact of high glucose on keratinocyte migration.
To assess the effects of high glucose on keratinocyte proliferation.
To explore the underlying molecular mechanisms affected by hyperglycemia in keratinocytes.

Main Methods:

Keratinocytes were cultured under normal and high glucose conditions.
Cell migration was assessed using Transwell and scratch assays.
Cell proliferation, viability, MMP/TIMP expression, and focal adhesion kinase signaling were evaluated.

Main Results:

High glucose did not affect keratinocyte viability within 3 days.
Hyperglycemia significantly reduced keratinocyte mobility, proliferation, MMP-2/MMP-9 activity, and pp125(FAK) expression after 5 days.
Increased mRNA expression of TIMP-1 was observed under high glucose conditions.

Conclusions:

Hyperglycemia significantly impairs keratinocyte migration and proliferation.
These functional deficits in keratinocytes lead to inadequate re-epithelialization.
The findings provide a cellular basis for the poor wound healing observed in patients with diabetes mellitus.