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[Gene expression profile of human TGF-beta signal transduction pathway of B cell type acute lymphocytic leukemia]

She-Dan Luo1, Yong Wu, Jian-Hua Chen

  • 1Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China.

Zhongguo Shi Yan Xue Ye Xue Za Zhi
|August 23, 2008
PubMed

This study was aimed to investigate the gene expression profile of TGF-beta signal transduction pathway in B-cell type acute lymphocytic leukemia (B-ALL). The gene expression profiles in B-ALL primary cells and cell lines (NALM6 cells, Raji cells), B-lymphocyte of control were detected by cDNA microarray including 113 different genes in human TGF-beta/BMP signal transduction pathway, and TGF-beta(1) mRNA expression was detected by real time RT-PCR. The B lymphocytes in peripheral blood of heacthy persons sorted by flow cytometry were used as control. The difference between them was compared. The results showed that as compared with B lymphocytes in peripheral blood of heacthy persons, the TGF-beta(1) expression in B-ALL cells, NALM6 cells and Raji cells were down-regulationed, myc and smad1 gene expressions were up-regulated, IL-6, smad 7 gene expressions were down-regulated. It is concluded that TGF-beta signal transduction is abnormal in B-cell type acute lymphocytic leukemia.

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