Related Experiment Video
Updated: Jul 2, 2026
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
A comparative study of histologic response to different pulp capping materials and a novel endodontic cement
Saeed Asgary1, Mohammad Jafar Eghbal, Masoud Parirokh
1Iranian Center for Endodontic Research, Dental Research Center, Shahid Beheshti University M.C., Tehran, Iran.
Objective:
The aim of this study was to compare dogs' pulp response to capping with calcium hydroxide cement (Dycal), mineral trioxide aggregate (MTA), and a novel endodontic cement (NEC).
Study Design:
Twenty-four canine teeth in 6 beagle dogs were buccocervically exposed and capped with MTA, Dycal, or NEC. Eight weeks later, retrieved samples were observed by optical microscope to grade inflammation, formation of dentinal bridge, calcification, necrosis, and presence of odontoblast cells.
Results:
No inflammation was observed in MTA and NEC groups, and in 75% of each, dentinal bridge was completely formed. In the Dycal group, inflammation and incomplete dentinal bridge were detected in all cases. Although MTA and NEC groups had no significant difference between them in each measure, the difference between all 3 groups was significant (P < .001).
Conclusion:
MTA and NEC showed similar favorable biologic response in pulp cap treatment, better than Dycal, especially in inducing the formation of dentinal bridge.
