Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Innovations in applications of mass spectrometry impact many fields of science.

Mass spectrometry reviews·2023
Same author

MALDI-TOF Mass Spectrometric Detection of SARS-CoV-2 Using Cellulose Sulfate Ester Enrichment and Hot Acid Treatment.

Journal of proteome research·2022
Same author

Molecular cargo in myeloid-derived suppressor cells and their exosomes.

Cellular immunology·2020
Same author

Extraction of Membrane Components from <i>Neisseria gonorrhoeae</i> Using Catanionic Surfactant Vesicles: A New Approach for the Study of Bacterial Surface Molecules.

Pharmaceutics·2020
Same author

Microwave supported hydrolysis prepares <i>Bacillus</i> spores for proteomic analysis.

International journal of mass spectrometry·2020
Same author

Analysis of the topology of ubiquitin chains.

Methods in enzymology·2019

Related Experiment Video

Updated: Jul 2, 2026

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

Mass spectrometry in biodefense.

Plamen A Demirev1, Catherine Fenselau

  • 1Applied Physics Laboratory, Johns Hopkins University, Laurel, MD 20723, USA. plamen.demirev@jhuapl.edu

Journal of Mass Spectrometry : JMS
|August 23, 2008
PubMed
Summary

Mass spectrometry (MS) rapidly identifies potential bioweapons by detecting protein biomarkers. This proteomics approach, aided by bioinformatics, enables swift and accurate detection for biodefense applications.

Area of Science:

  • Biodefense
  • Analytical Chemistry
  • Biochemistry

Background:

  • Potential agents for biological attacks include microorganisms and toxins.
  • Mass spectrometry (MS) is crucial for rapid identification of bioagents through unique biomarker detection.
  • Proteins are currently the most reliable biomarkers for microorganism and toxin detection and characterization.

Discussion:

  • MS-based proteomics is well-suited for biodefense due to its ability to identify individual protein biomarkers.
  • Top-down and bottom-up proteomics, coupled with MS/MS, provide confident and unambiguous bioagent identification.
  • Bioinformatics simplifies the process by eliminating the need for culturing and enabling the use of MS fingerprint libraries.

Key Insights:

  • Proteomics using mass spectrometry offers rapid and reliable identification of bioweapon agents.

More Related Videos

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
09:26

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

Related Experiment Videos

Last Updated: Jul 2, 2026

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
09:26

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

  • Both top-down and bottom-up proteomics strategies are effective for bioagent characterization.
  • Bioinformatics and MS fingerprint libraries enhance the efficiency of bioweapon detection.

    • Outlook:

    • Development of MS methods for identifying non-protein biomarkers is ongoing for specific applications.
    • Continued advancements in MS instrumentation and algorithms will further improve biodefense capabilities.
    • Integration of proteomics and bioinformatics is key to strengthening rapid response to biological threats.