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Updated: Jul 2, 2026

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
Published on: September 26, 2019
[Near infrared Raman spectra analysis of rhizoma dioscoreae]
Wen-Shuo Lin1, Rong Chen, Yong-Zeng Li
1Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China. lws43@163.com
Abstract:
A novel and compact near-infrared (NIR) Raman system was developed using 785 nm diode laser, volume-phase technology holographic system, and NIR intensified charge-coupled device (CCD). The Raman spectra and first derivative spectra of rhizoma dioscoreae were obtained. The Raman spectra of rhizoma dioscoreae showed three strong characteristic peaks at 477, 863 and 936 cm(-1), respectively. The major ingredients are protein, amino acid, starch, polysaccharides and so on, matching the known basic biochemical composition of rhizoma dioscoreae. In the first derivative spectra of rhizoma dioscoreae, the distinguishing characteristic peaks appeared at 467, 484, 870 and 943 cm(-1). Contrasted with rhizoma dioscoreae Raman spectra in the ranges of 600 to 800 cm(-1) and 1 000 to 1 400 cm(-1), the changes in rhizoma dioscoreae Raman first derivative spectra are represented more clearly than the rhizoma dioscoreae Raman spectra. So the rhizoma dioscoreae Raman first derivative spectra can be an accurate supplementary analysis method to the rhizoma dioscoreae Raman spectra.
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