[Optimization of plantaricin production by Lactobacillus plantarum ZJ316]

To enhance the production of plantaricin by Lactobacillus plantarum ZJ316 isolated from infant feces.

We analyzed fermentation parameters influencing cell growth and plantaricin production with different medium composition and under different cultivation conditions.

MRS (DE MAN, ROGOSA, SHARPE) medium was more suitable for producing bacteriocin than other media. The maximum plantaricin production was obtained in modified MRS medium containing 10 g/L maltose and 10 g/L glucose, 10 g/L yeast extract, 10 g/L tryptone and 2g/L tri-ammonium citrate, 1 mL/L Tween80, 6 g/L K2HPO4.3H2O, 5 g/L sodium acetate, 0.2 g/L magnesium sulfate and 0.05 g/L manganese sulfate. The optimal initial pH and temperature for plantaricin production were 6.5 and 30 degrees C for 24 h.

After optimizing, the production of plantaricin was increased 2.3-fold using the optimized medium, compared with in the basic MRS medium.