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[Authentication of Pinellia pedatisecta and their adulterants by AS-PCR]
Guang-Hong Cui1, Xiao-Jing Tang, Lu-Qi Huang
1Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing 100700, China.
Objective:
To establish a molecular method for the authentication of Pinellia pedatisecta and its adulterants.
Method:
DNA sequences of some species from P. tenore, Typhonium and Arisaema were downloaded from GenBank, the sequences were aligned using DNAMAN. Allele-specific primers for P. pedatisecta and P. tenore were designed according to their SNPs in rpl 20 sequence. The designed primers were used to amplify 10 samples of P. pedatisecta, P. ternata and T. flagelliforme.
Result:
A 351 bp band was amplified from P. pedatisecta but not form P. ternata and T. flagelliforme by primer Pprpl149F and Pprpl484R. A 630 bp band was amplified from P. ternate and P. pedatisecta but not from T. flagelliforme by primer Ptrpl94F and Ptrpl699R.
Conclusion:
AS-PCR has the advantages of highly specific and good reproducibility, by which P. pedatisecta can be identified from part of its adulterants quickly. It is a potential method to be used in the molecular identification of other materia medica.
