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Updated: Jul 2, 2026

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Temperature-sensitive nonionic vesicles prepared from Span 80 (sorbitan monooleate).

Keiichi Kato¹, Peter Walde, Norio Koine

¹Department of Applied Chemistry, Faculty of Engineering, Ehime University, Matsuyama, Japan. kato@eng.ehime-u.ac.jp

Langmuir : the ACS Journal of Surfaces and Colloids

|August 30, 2008

Summary

This summary is machine-generated.

Span 80 vesicles offer a cost-effective, biocompatible alternative to liposomes. These sorbitan monooleate vesicles are stable at room temperature but fuse irreversibly at higher temperatures, demonstrating thermoresponsive behavior.

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Published on: August 24, 2016

Area of Science:

Materials Science
Biotechnology
Physical Chemistry

Background:

Conventional phospholipid-based vesicles (liposomes) are widely used but can be expensive.
Nonionic surfactants offer potential alternatives for vesicle formulation.
Span 80 (sorbitan monooleate) is an inexpensive and biocompatible surfactant.

Purpose of the Study:

To prepare and characterize nonionic vesicles from Span 80.
To compare Span 80 vesicles with conventional phospholipid vesicles (POPC, DOPC).
To investigate the stability, thermoresponsiveness, and permeability of Span 80 vesicles.

Main Methods:

Vesicle preparation using Span 80, POPC, and DOPC.
Characterization via dynamic light scattering (DLS) and electron microscopy (EM).
Fusion assays, differential scanning calorimetry (DSC), and fluorescence probe (DPH) measurements.

Main Results:

Span 80 vesicles are stable for 7 days at 4°C and 25°C.
Irreversible vesicle fusion occurs at 42°C, indicating thermoresponsiveness.
Span 80 vesicles exhibit lower phase transition temperature (Tm < -30°C) and higher membrane fluidity than POPC/DOPC vesicles.
Higher fluidity correlates with increased leakage, which can be modulated by cholesterol and phosphatidylcholine.

Conclusions:

Span 80 vesicles are a stable, thermoresponsive alternative to liposomes.
Their tunable permeability offers potential for controlled release applications.
The thermoresponsive fusion property is linked to headgroup dehydration, not Tm.