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Updated: Jul 2, 2026
Technique of Conjunctival Biopsy and Direct Immunofluorescence for Diagnosing Mucous Membrane Pemphigoid
Published on: June 17, 2025
A study on plucked hair as a substrate for direct immunofluorescence in pemphigus vulgaris
M Daneshpazhooh1, M Asgari, Z S Naraghi
1Autoimmune Bullous Diseases Research Center, Department of Dermatology, School of Medicine, Medical Sciences/Tehran University, Tehran, Iran. daneshpj@sina.tums.ac.ir
Background:
It has recently been demonstrated in a study on 15 patients that plucked hair can be used as a substrate for direct immunofluorescence (DIF) in pemphigus.
Objective:
Our aim was to assess the sensitivity of DIF on plucked hairs in pemphigus vulgaris (PV) patients with positive DIF of oral mucosa.
Methods:
One hundred and ten new PV patients were enrolled in the study. They all showed the typical clinical and histological findings as well as positive DIF of the oral mucosa, diagnostic for PV. Approximately 30 hairs were obtained in the same way as for the trichogram. The hairs with their outer root sheaths (ORS) were processed for DIF in order to detect immunoglobulin G and C3.
Results:
Immunodeposits favouring PV were demonstrated in the ORS of 100 cases showing a sensitivity of 91%.
Conclusion:
Regarding the relatively high sensitivity of DIF on plucked hair in PV patients with positive oral mucosal DIF in our study, it seems that hair plucking is a suitable alternative to the more invasive techniques of skin or mucosal biopsy for obtaining specimens for DIF in PV.
