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Related Experiment Video

Updated: Jul 2, 2026

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Differential screening identifies transcripts with depot-dependent expression in white adipose tissues.

Yu Wu1, Ji Young Kim, Shengli Zhou

  • 1Department of Biochemistry and Cancer Biology, University of Toledo Health Science Campus, Toledo, OH 43614, USA. Yu.wu@utoledo.edu

BMC Genomics
|August 30, 2008
PubMed
Summary

Researchers identified distinct gene expression in white adipose tissue (WAT) depots, revealing depot-specific roles for Boc and Major Urinary Proteins (Mups) in obesity and adipogenesis.

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Area of Science:

  • Adipose tissue biology
  • Molecular endocrinology
  • Obesity research

Background:

  • Obesity comorbidities correlate with fat distribution in intra-abdominal versus subcutaneous white adipose tissue (WAT).
  • Distinct gene expression in WAT depots may confer depot-dependent physiological functions.

Purpose of the Study:

  • To identify genes with differential expression between murine subcutaneous (SC) and intra-abdominal epididymal (EP) white adipocytes.
  • To investigate the role of depot-specific transcripts in obesity and adipogenesis.

Main Methods:

  • Subtractive cDNA library preparation from murine SC and EP adipocytes.
  • Differential screening and quantitative PCR (qPCR) validation of identified transcripts.
  • Assessment of gene expression in various murine tissues, adipogenesis models, and during obesity.

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Last Updated: Jul 2, 2026

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
10:28

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

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Main Results:

  • Seven transcripts showed at least 2.5-fold enrichment in EP vs. SC adipocytes, with Boc (hedgehog pathway component) showing highest enrichment (~12-fold) in EP adipocytes.
  • Major Urinary Proteins (Mups) transcripts were highly enriched in SC WAT vs. EP WAT and increased significantly during preadipocyte differentiation.
  • Boc expression decreased in obese EP WAT but increased in obese SC WAT, while Mup transcripts were reduced in obese mice.

Conclusions:

  • Depot-specific gene expression in WAT may underlie the physiological impact of regional adiposity.
  • The Boc-Cdon axis may play a role in WAT depot function.
  • Further investigation of WAT depot-enriched transcripts can elucidate distinctions in gene expression and their physiological significance.