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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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GFP family: structural insights into spectral tuning

Alexey A Pakhomov1, Vladimir I Martynov

  • 1Chromoproteins Chemistry Research Group, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

Chemistry & Biology
|August 30, 2008
PubMed
Summary

Green fluorescent protein (GFP) homologs are vital for live cell imaging. Understanding their optical self-tuning mechanisms, like structural transformations, aids in engineering new variants and expanding their spectral range.

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