Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Accuracy of data routinely collected by Australian hospitals for identifying infective endocarditis, causative organisms and clinical outcomes: a diagnostic accuracy study.

Internal medicine journal·2026

Same author

Organization and Dynamics of Focal Adhesions: Light Diffraction Analysis of Cellular Adhesion on Nanopatterned Surfaces.

ACS applied materials & interfaces·2026

Same author

Therapeutic drug monitoring and the role of pharmacokinetics/pharmacodynamics in optimizing beta-lactam antibiotic dosing for deep-seated infections: a systematic review.

The Journal of antimicrobial chemotherapy·2026

Same author

Destructive <i>Erysipelothrix rhusiopathiae</i> Endocarditis Confirmed on Valve Tissue Culture After Persistent Blood Culture Negativity.

Clinical case reports·2026

Same author

Optical Waveguide Lightmode Spectroscopy: A Versatile Technique for Real-Time, Label-Free Biosensing.

Sensors (Basel, Switzerland)·2026

Same author

Correction: Kanyo et al. Kinetic Analysis of SARS-CoV-2 S1-Integrin Binding Using Live-Cell, Label-Free Optical Biosensing. <i>Biosensors</i> 2025, <i>15</i>, 534.

Biosensors·2026

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Multidepth screening of living cells using optical waveguides.

Robert Horvath¹, Kaspar Cottier, Henrik C Pedersen

¹Nanotechnology Centre, Cranfield University, Bedfordshire MK43 0AL, United Kingdom.

Biosensors & Bioelectronics

|August 30, 2008

Summary

This study demonstrates label-free cell monitoring using optical waveguides. Researchers developed a method to analyze cell refractive index profiles at specific depths, enabling non-invasive cell analysis.

More Related Videos

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Related Experiment Videos

Last Updated: Jul 2, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Area of Science:

Biophotonics
Cell Biology
Materials Science

Background:

Label-free, non-invasive cell monitoring is crucial for understanding cellular behavior.
Optical waveguides offer a platform for sensitive detection of biological samples.
Submicrometre resolution is needed to analyze cellular structures and dynamics.

Purpose of the Study:

To demonstrate the use of planar optical waveguides for label-free, non-invasive monitoring of cells.
To develop methods for quantitative analysis of cell refractive index profiles at submicrometre depths.
To enable real-time kinetic monitoring of cellular changes.

Main Methods:

Utilized a symmetry waveguide configuration with nanoporous silica and a polystyrene waveguiding film.
Employed a heat-embossed grating coupler to achieve distinct penetration depths for waveguide modes.
Developed robust data processing techniques to extract cell refractive index information from effective refractive indices.

Main Results:

Demonstrated selective monitoring at different submicrometre depths within cells.
Introduced a method using two modes to separate refractive index variations above and below a specific depth.
Showcased the ability to gain comprehensive information from multiple submicrometre slices of the cell layer.

Conclusions:

Planar optical waveguides provide a powerful tool for label-free, non-invasive cell monitoring.
The developed methods allow for quantitative analysis of cell refractive index profiles with depth resolution.
This technique facilitates the study of cellular dynamics and responses in real-time.