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Published on: October 12, 2017
Multicentric evaluation of the homogeneous LDL-cholesterol Plus assay: comparison with beta-quantification and
Margarita Esteban-Salan1, José Angel Aguilar-Doreste, Maria Luisa Arranz-Pena
1Laboratorio de Bioquímica, Hospital de Galdakao, Barrio Labeaga s/n. Galdakao, 48960 Vizcaya, Spain. MARGARITA.ESTEBANSALAN@osakidetza.net
Objectives:
To evaluate the analytical and clinical performance of a new version of the LDL-C Plus assay and compare it with the beta-quantification (BQ) method in a multicenter study.
Design And Methods:
Direct LDL-C was measured in 169 fresh pooled samples and in 830 clinical samples with known LDL-C by BQ. The reactivity of lipoproteins and the effect of hemoglobin, bilirubin and chylomicrons (CM) were studied.
Results:
Direct LDL-C total imprecision was <2.2%; inaccuracy <+/-2.5% (unaffected by triglycerides up to 9.5 mmol/L); and total error 9.8%. Direct assay reacted with 95%, 50% and 25% of the cholesterol in the LDL, intermediate (IDL) and VLDL fractions, respectively. A significant association was observed with BQ. Icteric samples showed a negative bias and the effect of CM was variable. A positive bias was observed when VLDL-cholesterol/triglyceride ratio was >0.57.
Conclusions:
LDL-C Plus assay represents a valid alternative to BQ for clinical laboratories.
