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Elimination of keratin artifact bands from western blots by using low concentrations of reducing agents

T-F Lee1, T W McNellis

  • 1Plant Pathology Department, 211 Buckhout Laboratory, The Pennsylvania State University, University Park, PA 16802, USA.

Analytical Biochemistry
|August 30, 2008
PubMed

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...