Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Selenalysine and protein synthesis.

C De Marco, V Busiello, M Di Girolamo

    Biochimica Et Biophysica Acta
    |December 1, 1976
    PubMed
    Summary

    Selenalysine, a lysine analog, inhibits protein synthesis by competing with lysine. While effective, its inhibitory activity is slightly lower than that of thialysine.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    HER2-low breast cancer is immune-cold: insights into tumor-infiltrating immune cells and implications for immunotherapy.

    NPJ breast cancer·2025
    Same author

    Effects of Microminerals on Performance and Metabolic Adaptation in Heat-Stressed Dairy Cows: A Review.

    Biological trace element research·2025
    Same author

    Time-activity budget in horses and ponies: A systematic review and meta-analysis on feeding dynamics and management implications.

    Journal of equine veterinary science·2025
    Same author

    Artificial intelligence meets dairy cow research: Large language model's application in extracting daily time-activity budget data for a meta-analytical study.

    Journal of dairy science·2025
    Same author

    Atrial depolarization electrocardiographic features during orthodromic atrioventricular reciprocating tachycardia mediated by right-sided accessory pathway in the dog.

    Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology·2025
    Same author

    Reliability of core needle biopsy for HER2-low early-stage breast cancer.

    Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2025

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Amino Acid Analogs

    Background:

    • Lysine is an essential amino acid crucial for protein synthesis.
    • Amino acid analogs can interfere with biological processes.
    • Thialysine is a known lysine analog used for comparison.

    Purpose of the Study:

    • To investigate the biochemical properties and inhibitory effects of selenalysine.
    • To compare the activity of selenalysine with thialysine in protein synthesis.

    Main Methods:

    • Activation and transfer of selenalysine to tRNAlys using aminoacyl-tRNA synthetases from E. coli and rat liver.
    • Assaying inhibition of lysine incorporation into polypeptides in cell-free protein synthesis systems.
    • Comparative analysis with thialysine.

    Main Results:

    • Selenalysine is activated and transferred to tRNAlys by aminoacyl-tRNA synthetases.
    • Selenalysine inhibits lysine incorporation into polypeptides in various protein-synthesizing systems.
    • Both thialysine and selenalysine act as competitive inhibitors of lysine.
    • Selenalysine exhibits slightly lower inhibitory activity compared to thialysine.

    Conclusions:

    • Selenalysine functions as a competitive inhibitor of lysine in protein synthesis.
    • The selenium substitution in selenalysine impacts its inhibitory potency relative to thialysine.

    Related Experiment Videos