Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transcription Attenuation in Prokaryotes

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...

Termination of Translation

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...

Termination of Translation

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...

Eukaryotic RNA Polymerases

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...

Eukaryotic RNA Polymerases

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...

Replication in Eukaryotes

Replication in Eukaryotes

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cardiac mesenchymal stromal cells are a source of adipocytes in arrhythmogenic cardiomyopathy.

European heart journal·2015

Same author

High concordance of genomic and cytogenetic aberrations between peripheral blood and bone marrow in myelodysplastic syndrome (MDS).

Leukemia·2015

Same author

Anaplastic large cell lymphoma-propagating cells are detectable by side population analysis and possess an expression profile reflective of a primitive origin.

Oncogene·2014

Same author

A clinical decision rule to enhance targeted bone mineral density testing in healthy mid-life women.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2011

Same author

Photoinduced work function changes by isomerization of a densely packed azobenzene-based SAM on Au: a joint experimental and theoretical study.

Physical chemistry chemical physics : PCCP·2011

Same author

Osteoarthritis year 2010 in review: non-pharmacologic therapy.

Osteoarthritis and cartilage·2011

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Published on: March 12, 2017

Rtf1-mediated eukaryotic site-specific replication termination.

T Eydmann¹, E Sommariva, T Inagawa

¹Marie Curie Research Institute, The Chart, Oxted RH8 0TL, United Kingdom.

|August 30, 2008

Summary

This summary is machine-generated.

Replication termination in eukaryotes is clarified by characterizing Rtf1, a protein essential for site-specific DNA replication pausing at the RTS1 barrier in fission yeast. Rtf1

More Related Videos

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

Related Experiment Videos

Last Updated: Jul 2, 2026

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Published on: March 12, 2017

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Eukaryotic DNA replication termination and pausing mechanisms are not fully understood.
Replication termination ensures genome stability by preventing conflicts between replication and transcription machinery.

Purpose of the Study:

To elucidate the molecular mechanisms of site-specific replication termination.
To characterize the role of the Rtf1 protein in replication termination at the RTS1 barrier in *Schizosaccharomyces pombe*.

Main Methods:

Molecular characterization of the Rtf1 protein.
Analysis of Rtf1's DNA-binding and protein-protein interaction domains.
Site-directed mutagenesis to investigate Rtf1 function and polarity determination.

Main Results:

Rtf1 possesses two chimeric myb/SANT domains, with one mediating interaction with RTS1 element motifs and enhancer regions.
The C-terminal tail of Rtf1 is crucial for self-interaction, and its deletion results in a dominant phenotype.
A specific point mutation in Rtf1 domain I reverses the polarity of the RTS1 replication barrier.

Conclusions:

Multiple DNA and protein-protein interactions involving Rtf1, RTS1 motifs, and enhancer regions are essential for site-specific replication termination.
Rtf1 acts as a key mediator of replication termination, integrating DNA sequence information and protein complex formation.
The study provides critical insights into the regulation of DNA replication fork progression and termination in eukaryotes.