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A Murine Orthotopic Bladder Tumor Model and Tumor Detection System

A Murine Orthotopic Bladder Tumor Model and Tumor Detection System

Published on: January 12, 2017

Bladder cancer detection using FISH (UroVysion assay).

Kevin C Halling¹, Benjamin R Kipp

¹Department of Laboratory Medicine and Pathology, Division of Laboratory Genetics, Mayo Clinic and Foundation, Rochester, MN 5905, USA. halling.kevin@mayo.edu

Advances in Anatomic Pathology

|August 30, 2008

Summary

The UroVysion assay detects bladder cancer by identifying chromosomal abnormalities in urine. This fluorescence in situ hybridization test is more sensitive than urine cytology for detecting bladder cancer.

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Area of Science:

Urology
Oncology
Genetics

Background:

Bladder cancer diagnosis relies on various methods, including urine cytology.
Urine cytology has limitations in sensitivity for detecting bladder cancer at different stages and grades.
There is a need for more sensitive diagnostic tools for bladder cancer detection.

Purpose of the Study:

To evaluate the UroVysion fluorescence in situ hybridization (FISH) assay for bladder cancer detection.
To compare the sensitivity of UroVysion assay with urine cytology.
To explore potential applications of UroVysion in monitoring treatment response and detecting upper tract urothelial carcinoma.

Main Methods:

UroVysion assay utilizes fluorescently labeled DNA probes targeting chromosomes 3, 7, 17, and the 9p21 band (P16 gene).

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A Murine Orthotopic Bladder Tumor Model and Tumor Detection System

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The assay detects chromosomal abnormalities in exfoliated urothelial cells in urine specimens.

Comparison with urine cytology was performed to assess diagnostic accuracy.

Main Results:

UroVysion demonstrated higher sensitivity than urine cytology for detecting all stages and grades of bladder cancer.
The assay is FDA-approved for detecting recurrent bladder cancer and for initial diagnosis in patients with hematuria.
Emerging evidence suggests utility in assessing bacillus Calmette-Guerin therapy response and detecting upper tract urothelial carcinoma.

Conclusions:

UroVysion is a sensitive and effective tool for bladder cancer detection in urine specimens.
The assay offers advantages over traditional urine cytology, particularly for recurrent and newly diagnosed bladder cancer.
Further research indicates UroVysion's potential in broader urothelial carcinoma management.