Related Experiment Videos
Mass spectrometric signature of S-prenylated cysteine peptides
A A Tuinman1, D A Thomas, K D Cook
1Department of Chemistry, University of Tennessee, Knoxville 37996.
Analytical Biochemistry
|March 2, 1991
Abstract:
The fast atom bombardment mass spectra of peptides containing S-prenylated cysteine display signature fragmentations characteristic of this modified amino acid. The fragmentation is independent of the nature of the cysteine carbonyl substituent, easily differentiates prenyl from nonprenyl alkylation, and readily identifies the oligomer count of the prenyl. This screening method, which requires little time, effort, or material (compared with previous analysis methods based on chemical degradation), greatly facilitates the identification of these prenylated proteins.