[Developments of microfluidic chip-based capillary electrophoresis for protein separation]

Area of Science:

• Analytical Chemistry
• Biochemistry
• Microfluidics

Context:

• Protein separation is crucial for proteomics and diagnostics.
• Traditional methods face challenges in speed, sensitivity, and sample consumption.
• Microfluidic chip-based capillary electrophoresis (CE) offers a miniaturized and efficient alternative.

Purpose:

• To review recent developments in microfluidic chip-based CE for protein separation.
• To introduce various chip-based CE systems and separation modes.
• To discuss strategies for mitigating protein adsorption on microchannels.

Summary:

• This paper reviews the latest advancements in microfluidic chip-based capillary electrophoresis (CE) for protein separation.
• It details different chip-based CE systems and separation techniques.
• Methods to minimize protein adsorption onto microchannel surfaces are explored.

Impact:

• Highlights the potential of chip-based CE for high-throughput and sensitive protein analysis.
• Provides insights into overcoming technical challenges in microfluidic protein separations.
• Suggests future research directions and applications in proteomics and clinical diagnostics.