[Separation and purification of porcine hemoglobin from porcine blood]

In order to perform the isolation and purification of porcine hemoglobin from porcine blood. A three-step method, utilizing ultrafiltration, DEAE-Sepharose Fast Flow anion-exchange chromatography and Sephadex G-75 gel exclusion chromatography in sequence, for the separation and purification of porcine hemoglobin was developed. The obtained hemoglobin was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), high-performance size-exclusion chromatography and reversed-phase high performance liquid chromatography. Through the three-step purification process, the purity of the obtained hemoglobin was more than 99% with a concentration of 1.328 g/L.