[Determination of triazolam in human urine by gas chromatograph-micro electron capture detector]

Triazolam is often abused in criminal activities. It is necessary to develop a simple, fast and sensitive method for determining triazolam in human urine. After the triazolam in urine was extracted with ether at pH 9, the analysis was performed on an HP-5 capillary column with gas chromatograph-micro electron capture detector (GC-muECD). The calibration curve of triazolam showed good linearity in the range of 0.2 - 50 ng/mL with the correlation coefficient of 0.999 5. The inter-day relative standard deviation (RSD) and intra-day RSD were 4.17% and 5.31%, respectively. The average recovery was 93.9%. This simple, sensitive and accurate method can be easily implemented for monitoring triazolam in urine.