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Assay of HIV-1 proteinase: a colorimetric method using small peptide substrates
A V Broadhurst1, N A Roberts, A J Ritchie
1Department of Chemotherapy Biology, Roche Products Ltd., Welwyn Garden City, Hertfordshire, United Kingdom.
Analytical Biochemistry
|March 2, 1991
Abstract:
A colorimetric assay for HIV proteinase using small protected peptide substrates is described. Substrates are cleaved to release N-terminal prolyl peptides which react with isatin to form a blue product which is measured spectrophotometrically. The assay is suitable for use with pure enzyme or crude extracts derived from genetically engineered Escherichia coli.